|
NucIt™ is a multi-function web-based program. It can aid in
identifying optimal oligonucleotide primers within a specified target
DNA/RNA sequence, calculate DNA duplex melting temperature (Tm ), search
for specified motifs in a target, find repeating sequences in a target,
inverse complement a target sequence, translate a DNA sequence and
reverse translate an amino acid sequence and identify degenerate
oligonucleotide primers. NucIt™ will also evaluate oligonucleotides
for their tendency to self-dimerize or form a duplex with another
oligonucleotide, to form hairpin structures and to false prime other
sites in a given target sequence. NucIt™ will help take the
guesswork out of primer design and thus reduce or eliminate the
occurrence of many spurious primer-initiated products in polymerase
amplification and DNA sequencing as well as aide in the design of
oligonucleotide probes for hybridization analysis.
NucIt™ Batch siRNA Duplex Design is a web-based tool for searching multiple target sequences for candidate siRNA sequences having the AA19NTT or AA21N motif. The program is designed for batch entry of any number of query sequences. siRNAs identified by this program are based on parameters described by Elbashir et al. (2001). Candidate siRNA sequences are hyperlinked to enable BLAST searching and the algorithm flags potentially problematic siRNA sequences having polyG, C, A, or T nucleotides. Use of NucIt Batch siRNA Duplex Design requires a license. Please inquire for pricing. |